Routine blood is a common inspection item in hospitals, but because most hospitals use three-category hemocytometers (the price of five-category hemocytometers is too high), there are certain defects in analysis, while blood smears can be used in blood smears.
Blood smear production:
There are many ways to make blood smears, but the materials required are the same, glass slides and/or coverslips.
1. First, hold a glass slide in the left hand, and drop the collected blood (the blood left from the blood routine) to one end of the glass slide:
blood smear
2. Hold a cover glass in the right hand as a pusher, at a 30-45 degree angle in front of the blood drop and close to the blood drop
blood smear
3. Move the coverslip with the right hand so that one end is in contact with the blood
blood smear
4. Push the cover glass forward with the right hand, and the speed should not be too slow to ensure that the blood is evenly pushed away
blood smear
5. Prepared blood smear
blood smear
5. After the blood smear is made, it needs to be air-dried, and then it can be stained. Although the staining solution used in each hospital may be different, the basic methods are the same. You can also perform specific operations according to the instructions of the staining solution.
The dyed film needs to be observed under a microscope. Generally, we will start with a low-power lens and then gradually switch to a high-power lens or even an oil lens.
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Cell Sorting Counter
Under normal circumstances, we will find a suitable field of view under the microscope to start counting, and count the white blood cells in a certain direction and order, and the selection of this field of view will be very important.
Cell Sorting Counter
(Cell Sorting Counter)
Through this method, we can get the proportion of various types of leukocytes, and combine with the number of leukocytes in the blood routine to get a five-category item, which is more comprehensive and scientific for analyzing the physical condition of animals.
Let me give a simple example about the clinical application of blood smear, which is also combined with the analysis of blood routine.
From the results, RBC (red blood cells) decreased, HGB (hemoglobin) decreased, and HCT (hematocrit) decreased.
From the blood smear, we can see that the indifferent area in the middle of the red blood cells is obviously enlarged, indicating that this is normocytic hypochromic anemia, that is, due to iron deficiency anemia, iron supplementation is required to enhance nutrition.
Of course, the clinical role of blood smears is not only that, we can not only count and classify white blood cells, but also observe the shape of red blood cells to judge the reasons for the appearance of heterosexual red blood cells and abnormal white blood cells.